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AC 503 LABORATORY TECHNIQUES                                                                            (1L +2P) I

Objective
  To acquaint students with laboratory hygiene, upkeep and maintenance of laboratory, glassware and handling of chemicals, purification and drying of solvents, distillation and chromatographic techniques.

Theory

UNIT I
  Acquaintance with laboratory glassware and apparatus and laboratory safety procedures

UNIT II
  Solvent purification, drying and standard solutions

UNIT III
  Extraction techniques, distillation, crystallization, sublimation, separation methods

UNIT IV
  Functions of common equipments – water and oil pumps, heating and cooling baths, stirrers, rotary evaporators

UNIT V
  Principles of chromatography: thin layer, paper and column chromatography.

UNIT VI
  pH (Hydrogen ion concentration), electrical conductivity, electrophoresis, uv-vis spectrophotometry

Practicals
  Introduction to Laboratory equipment and cleaning of glassware, Assembling of simple apparatus and finding density, Purification of  solvents, Crystallization, identification and sublimation, Extraction, Chromatography: Paper, Column, TLC, Preparative TLC Column, Use of pH meters, electrical conductometer, electrophoresis, UV-Vis spectrophotometer, Steam Distillation, use of stirrer and vacuum pumps.

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