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AC 510 PESTICIDE RESIDUE CHEMISTRY                                                                            (3L+2P) III

Objective
  To teach students the concept of pesticide residue, planning, layout and design of experiments, instruments and techniques involved data analysis and legal issues of pesticide residue analysis.

Theory

UNIT I
  Pesticide residues-concepts and toxicological significance

UNIT II
  Experimental design and sampling

UNIT III
  Principles of extraction and clean up from different substrates

UNIT IV
  Application of spectrophotometric and chromatographic techniques estimation /detection of micro level pesticides residue, ELISA and radiotracer techniques

UNIT V
  Confirmatory techniques, bound and conjugated residues, multi residue methods

UNIT VI
  Method validation: linearity, accuracy, precision, LOD and LOQ. Interpretation of data and statistical analysis and measurement of uncertainty.

Practicals
  Identification of  Organochlorine insecticides in water by TLC, identification of  carbamate insecticides in water by TLC, estimation of carbamate insecticide residues in vegetables by visible spectroscopic method, estimation of organophosphorus insecticide residues in soil by visible spectroscopic method. Standardization of protocol for the detection of organochlorine / organophosphor us / carbamates pesticides by thin layer chromatography. Detection of organochlorine / organophosphorus / carbamates pesticides in water by thin layer chromatography and use of  suitable visualizing reagents. Standardization of  protocol for the detection of organophosphorus / carbamates by visible spectrophotometry- development of colored products. Extraction and clean up techniques for pesticides in different substrates,like water,milk, soil, vegetables and cereals. Use of UV, IR, GLC, HPLC and GC MS techniquies for estimation and confirmation of pesticide residues.

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