DESCRIPTION OF COURSES
AC 510 PESTICIDE RESIDUE CHEMISTRY (3L+2P) III
To teach students the concept of pesticide residue, planning, layout and design of experiments, instruments and techniques involved data analysis and legal issues of pesticide residue analysis.
Pesticide residues-concepts and toxicological significance
Experimental design and sampling
Principles of extraction and clean up from different substrates
Application of spectrophotometric and chromatographic techniques estimation /detection of micro level pesticides residue, ELISA and radiotracer techniques
Confirmatory techniques, bound and conjugated residues, multi residue methods
Method validation: linearity, accuracy, precision, LOD and LOQ. Interpretation of data and statistical analysis and measurement of uncertainty.
Identification of Organochlorine insecticides in water by TLC, identification of carbamate insecticides in water by TLC, estimation of carbamate insecticide residues in vegetables by visible spectroscopic method, estimation of organophosphorus insecticide residues in soil by visible spectroscopic method. Standardization of protocol for the detection of organochlorine / organophosphor us / carbamates pesticides by thin layer chromatography. Detection of organochlorine / organophosphorus / carbamates pesticides in water by thin layer chromatography and use of suitable visualizing reagents. Standardization of protocol for the detection of organophosphorus / carbamates by visible spectrophotometry- development of colored products. Extraction and clean up techniques for pesticides in different substrates,like water,milk, soil, vegetables and cereals. Use of UV, IR, GLC, HPLC and GC MS techniquies for estimation and confirmation of pesticide residues.