DESCRIPTION OF COURSES
AP 520 PRINCIPLES OF BIOPHYSICS (3L+1P) II
To impart theoretical and practical knowledge of different life forms, and interactive effects of various physical forces on life processes.
Introduction and scope of biophysics, weak and strong interactions in biological systems, structure and property of water, physical, chemical and biological origin of life
Experimental techniques used for separation and characterization of biomolecules: sedimentation, ultra-centrifugation, diffusion, osmosis, viscosity, polarization and electrophoresis, chromatography, aminoacid and nucleotide sequence analysis
Spectroscopic techniques for biomolecular characterization: UV-Visible, IR, NMR, EPR spectroscopy, X-ray diffraction & its application in biology
Structure and function of biological molecules: Amino acids and peptides, protein structure, nucleic acids, protein synthesis, mechanism of genetic control, polysaccharides and lipids-structure and function
Biomembranes-structure and function of plant and animal cell membranes, basis for cell membrane voltages, bioelectricity of cell membrane and its measurement, artificial membranes, transport phenomena in biological systems, active and passive transport
Structure and function of plant and animal cells and viruses, unicellular and multi-cellular life forms, types of specialized cells and their functions, cell to cell communication
Bio-energetic- First and second laws of thermodynamics, heat, work, entropy and free energy, concept of negative entropy & its application in living systems, information theory.
Spectroscopy-Verification of Beer-Lambert’s law, Spectroscopy-Absorption spectrum of chlorophyll a &b, Viscometer-Measurement of intrinsic viscosity and molecular mass, Polarimeter-Measurement of Molar rotation, Reftractometer-Measurement of specific and molar refractivity, NMR spectroscopy- Relaxation time measurements, Isolation of plant DNA Melting point of DNA, Electrophoresis of DNA/Protein