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AP 520 PRINCIPLES OF BIOPHYSICS                                                                                 (3L+1P) II

Objective
  To impart theoretical and practical knowledge of different life forms, and interactive effects of various physical forces on life processes.

Theory

UNIT I
  Introduction and scope of biophysics, weak and strong interactions in biological systems, structure and property of water, physical, chemical and biological origin of life

UNIT II
  Experimental techniques used for separation and characterization of biomolecules: sedimentation, ultra-centrifugation, diffusion, osmosis, viscosity, polarization and electrophoresis, chromatography, aminoacid and nucleotide sequence analysis

UNIT III
  Spectroscopic techniques for biomolecular characterization: UV-Visible, IR, NMR, EPR spectroscopy, X-ray diffraction & its application in biology

UNIT IV
  Structure and function of biological molecules: Amino acids and peptides, protein structure, nucleic acids, protein synthesis, mechanism of genetic control, polysaccharides and lipids-structure and function

UNIT V
  Biomembranes-structure and function of plant and animal cell membranes, basis for cell membrane voltages, bioelectricity of cell membrane and its measurement, artificial membranes, transport phenomena in biological systems, active and passive transport

UNIT VI
  Structure and function of plant and animal cells and viruses, unicellular and multi-cellular life forms, types of specialized cells and their functions, cell to cell communication

UNIT VII
  Bio-energetic- First and second laws of thermodynamics, heat, work, entropy and free energy, concept of negative entropy & its application in living systems, information theory.

Practicals
  Spectroscopy-Verification of Beer-Lambert’s law, Spectroscopy-Absorption spectrum of chlorophyll a &b, Viscometer-Measurement of intrinsic viscosity and molecular mass, Polarimeter-Measurement of  Molar rotation, Reftractometer-Measurement of  specific and molar refractivity, NMR spectroscopy- Relaxation time measurements, Isolation of plant DNA Melting point of DNA, Electrophoresis of DNA/Protein

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