DESCRIPTION OF COURSES
BIO 501 BASIC BIOCHEMISTRY (4L+1P) I
To provide basic knowledge/overview of structure and functional and metabolism of biomolecules.
Scope and importance of Biochemistry in Agriculture; Fundamental principles governing life; Structure and properties of water; Acid base concepts, pH and buffers; Intra- & inter-molecular forces in biomolecules; General introduction to physical techniques for determination of structure of biopolymers.
Classification, structure and function of carbohydrates, lipids, amino acids, proteins, nucleic acids and vitamins.
Fundamentals of thermodynamic principles applicable to biological process, bioenergetics; respiration and oxidative phosphorylation.
Classification of enzymes and their mechanism of action, regulation and kinetics.
Plant and animal hormones; Metabolism of carbohydrates, lipids & proteins, DNA replication, transcription and translation.
Preparation of reagents and buffers, Preparation of standard acids and alkali, Estimation of protein, free amino acids, estimation of amylolytic activity, Assay of proteolytic activity, Estimation of total sugars, Reducing sugars, Non reducing sugars, starch, Extraction and estimation of oil, Iodine value, Acid value, Fatty acid by GLC, Estimation of Vitamin C, Estimation of DNA, RNA.