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BIO 501 BASIC BIOCHEMISTRY                                                                                   (4L+1P) I

Objective
  To provide basic knowledge/overview of structure and functional and metabolism of biomolecules.

Theory

UNIT I
  Scope and importance of Biochemistry in Agriculture; Fundamental principles governing life; Structure and properties of water; Acid base concepts, pH and buffers; Intra- & inter-molecular forces in biomolecules; General introduction to physical techniques for determination of structure of biopolymers.

UNIT II
  Classification, structure and function of carbohydrates, lipids, amino acids, proteins, nucleic acids and vitamins.

UNIT III
  Fundamentals of  thermodynamic principles applicable to biological process, bioenergetics; respiration and oxidative phosphorylation.

UNIT IV
  Classification of enzymes and their mechanism of action, regulation and kinetics.

UNIT V
  Plant and animal hormones; Metabolism of carbohydrates, lipids & proteins, DNA replication, transcription and translation.

Practicals
  Preparation of reagents and buffers, Preparation of standard acids and alkali, Estimation of protein, free amino acids, estimation of amylolytic activity, Assay of proteolytic activity, Estimation of total sugars, Reducing sugars, Non reducing sugars, starch, Extraction and estimation of oil, Iodine value, Acid value, Fatty acid by GLC, Estimation of Vitamin C, Estimation of DNA, RNA.

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