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BIO 503 PLANT BIOCHEMISTRY                                                                               (3L+2P) II

Objective
  To provide insight into various biochemical and molecular processes in plants and their regulation

Theory

UNIT I
  Structure and function of plant cell organelles; Photosynthesis in higher plants; Light and Dark reactions; C3, C4 & CAM pathways and their regulation.

UNIT II
  Biosynthesis of structural carbohydrates, storage proteins and lipids; Sucrose-Starch interconversion; Photorespiration.

UNIT III
  Proteins: post-translational modifications, folding, stability, transport and degradation.

UNIT IV
  Cellular metabolism of oligosaccharides and polysaccharides; Secondary metabolites- isoprenoids, alkaloids and phenolics, cyanogenic glycosides and glucosinolates

UNIT V
  Biochemistry of Plant hormones; signaling pathways; Phytochromes.

Practicals
  Soluble Protein Estimation by Lowry’s Method; Fractionation of Cell Organelles; Estimation of Starch; Assay of ADPG Pyrophosphorylase; Assay of Starch Synthase; Estimation of Amylolytic Activity; Assay of Isocitrate Lyase; Assay of Isocitrate Dehydrogenase; Assay of Ribulose-1,5- Bisphosphate Carboxylase; Assay of Phenylalanine Ammonia Lyase; Assay of Pectin Methyl Esterase; Assay of Superoxide Dismutase; Assay of Polyphenol Oxidase; Assay of Lipoxygenase; Estimation of Proline; Estimation of Carotenoid Pigments; Determination of Polyphenols in Pulse Grains, Fractionations of storage proteins

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