DESCRIPTION OF COURSES
BIO 504 TECHNIQUES IN BIOCHEMISTRY (2L +2P) III
To impart theoretical and practical knowledge about various biochemical techniques used in biochemistry for isolation and characterization of cellular components.
rinciples of enzyme assays and analysis of biomolecules; Centrifugation techniques-theory, types and applications.
Principles and applications of Spectrophotometry, Mass spectroscopy.
Chromatographic techniques-TLC, ion - exchange, gel - filtration, affinity, GLC and HPLC.
Electrophoresis techniques – native and SDS-PAGE, Isoelectric focusing, capillary electrophoresis and agarose gel electrophoresis.
Concept of radioactivity, tracer techniques and their applications in biology, Scintillation counters, autoradiography, radiation hazards and safety measures.
Polymerase Chain Reaction (PCR); Immunochemical techniques, MALDI-TOF.
Ethical issues and professional responsibilities in research.
pH and buffer preparation; Fractionation of cell organelles; Soluble protein estimation by Lowry’s Method; Isolation, purification and distribution of polysomes; Gel Chromatography; polyacrylamide gel electrophoresis; Separation of proteins by iso-electric focusing. Isolation of plant DNA, Agarose gel electrophoresis; Estimation of fatty acids by gas-liquid chromatography, Radioisotope analysis by liquid scintillation counter, PCR.