DESCRIPTION OF COURSES

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BIO 601 NUCLEIC ACIDS                                                         (2L+1P) I

Objective
  To provide knowledge about structure and function of nucleic acids and the role of modification enzymes

Theory

UNIT I
  History of nucleic acids; DNA as genetic material.

UNIT II
  Chemistry of nucleic acids; Chromatin structure and function; Structure and conformation of DNA and RNAs.

UNIT III
  DNA topoisomerases, nucleases, endonucleases and related enzymes.

UNIT IV
  Biosynthesis of nitrogenous bases, nucleotides and their regulation.

Practicals
  Isolation and purification of RNA and DNA from plant tissue; Spectrophotometric analysis and agarose gel electrophoresis; Restriction analysis of DNA; Isolation of total RNA and Ribosomal RNA; Spectrophotometric quantification; agarose gel electrophoresis; Isolation of messenger RNA.

Suggested Readings