DESCRIPTION OF COURSES
BIO 601 NUCLEIC ACIDS (2L+1P) I
To provide knowledge about structure and function of nucleic acids and the role of modification enzymes
History of nucleic acids; DNA as genetic material.
Chemistry of nucleic acids; Chromatin structure and function; Structure and conformation of DNA and RNAs.
DNA topoisomerases, nucleases, endonucleases and related enzymes.
Biosynthesis of nitrogenous bases, nucleotides and their regulation.
Isolation and purification of RNA and DNA from plant tissue; Spectrophotometric analysis and agarose gel electrophoresis; Restriction analysis of DNA; Isolation of total RNA and Ribosomal RNA; Spectrophotometric quantification; agarose gel electrophoresis; Isolation of messenger RNA.