DESCRIPTION OF COURSES
BIO 603 GENETIC ENGINEERING – PRINCIPLES AND METHODS (3L +1P) I
To provide knowledge about the basics of recombinant DNA technology and its applications
Introduction and historical perspectives; Properties and applications of Restriction Enzymes; Characteristics of vectors; DNA cloning strategies.
Methods of gene isolation, Construction and screening of genomic and cDNA libraries, PCR; Site directed mutagenesis; Gene silencing Methodologies:–Anti-sense RNA Technology, Ribozyme technology; DNA sequencing.
Plant transformation methods and transgene analysis, Potential applications of genetic engineering in agriculture; Transcriptional and post transcriptional gene silencing, gene targeting and gene therapy.
Bioethics and biosafety issues and IPR in Recombinant DNA research.
Isolation of Plant, plasmid and phage DNA and purification; Restriction of Plant DNA; Southern blotting; Elution of DNA from agarose gel; Radioactive labeling of DNA; Southern hybridization, autoradiography. Preparation of vector for cloning; Ligation of vector with insert DNA; Preparation of competent cells; Transformation of E.coli cells and selection of recombinants by antibiotic or blue/white selection; PCR, Preparation of plant RNA, RT-PCR.