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BIO 605 ENZYMOLOGY                                                                                 (3L+2P) III

Objective
  To impart knowledge about the catalytic role of enzymes, their structure, Physico-chemical, kinetic and regulatory properties, mechanism of action and their importance in agriculture and allied sectors.

Theory

UNIT I
  Enzymes: str ucture and conformation; classification, assay, isolation, purification and characterization.

UNIT II
  Specificity, mechanism of action, steady state and pre-steady state kinetics; Active site mapping, regulation and enzyme activity.

UNIT III
  Immobilized enzymes and their applications.

UNIT IV
  Enzyme engineering in Agriculture and allied sectors.

Practicals
  Soluble protein Estimation by Lowry’s Method; Estimation of  Urease activity in Soybean; Cr ystallization of  Urease from soybean meal; Alter nate method for Urease extraction; Determination of pH optima for Urease ; Determination of the optimum temperature Q10  value and activation energy of Urease ; Study the effect of substrate concentration on enzymatic activity of Urease and determination of K and V Study of the effect of inhibitor on the activity of Urease; Estimation of ADH and alkaline phosphatase activities; Purification of peroxidase from wheat seedlings.

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