DESCRIPTION OF COURSES
BIO 605 ENZYMOLOGY (3L+2P) III
To impart knowledge about the catalytic role of enzymes, their structure, Physico-chemical, kinetic and regulatory properties, mechanism of action and their importance in agriculture and allied sectors.
Enzymes: str ucture and conformation; classification, assay, isolation, purification and characterization.
Specificity, mechanism of action, steady state and pre-steady state kinetics; Active site mapping, regulation and enzyme activity.
Immobilized enzymes and their applications.
Enzyme engineering in Agriculture and allied sectors.
Soluble protein Estimation by Lowry’s Method; Estimation of Urease activity in Soybean; Cr ystallization of Urease from soybean meal; Alter nate method for Urease extraction; Determination of pH optima for Urease ; Determination of the optimum temperature Q10 value and activation energy of Urease ; Study the effect of substrate concentration on enzymatic activity of Urease and determination of K and V Study of the effect of inhibitor on the activity of Urease; Estimation of ADH and alkaline phosphatase activities; Purification of peroxidase from wheat seedlings.