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BIO 607  INORGANIC NITROGEN METABOLISM                                                                              (3L+1P)

Objective
  To impart knowledge of biochemistry and molecular mechanism of biological nitrogen fixation mechanism and regulation of nitrate assimilation and denitrification processes.

Theory

UNIT I
  Biochemistry of nitrogen cycle. Biological nitrogen fixation; Structure, function and regulation of nitrogenase; Structure, function and regulation of nif genes in Klebsiella pneumoniae and Clostridium.

UNIT II
  Biochemical basis of legume-Rhizohium symbiosis; Genes involved in symbiosis. Different types of hydrogenases and role of uptake hydrogenase in N - fixation; Chemoautotrophy in rhizobia. Biochemistry of ferredoxin and other non-haem iron proteins.

UNIT III
  Biochemistry of nitrate assimilation and mechanism of its regulation; GS/GOGAT and GDH pathways; Ureides and amides as nitrogen transport compounds.

UNIT IV
  Biochemistry of denitrification process and phosphorylation in denitrifying bacteria. Path of carbon assimilation in nitrifying bacteria.

Practicals
  Estimation of nitrate content by hydrazine sulphate reduction method; Estimation of protein by Lowry’s Method; In vivo assay of nitrate reductase activity; In vitro assay of nitrite reductase activity; In vitro assay of glutamine synthetase activity; In vitro assay of glutamate synthase activity; In vitro assay of glutamate dehydrogenase activity; Assay of nitrogenase activity by acetylene reduction method; Estimation of hydrogen evolved by legume nodules.

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