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ENT 601 INSECT PATHOLOGY                                                                                 (2L+1P) III

Objective
To teach the students about various microbes that are pathogenic to insects, factors that affect their virulence, provide hands-on training in identification, isolation, culturing various pathogens and assessing pathogenicity.

Theory

UNIT I
History of insect pathology, infection of insects by bacteria, fungi, viruses, protozoa, rickettsiae, spiroplasma, and nematodes.

UNIT II
Epizootiology, symptomatology and etiology of diseases caused by the above, and the factors controlling these. Defense mechanisms in insects against pathogens.

UNIT II
Examples of successful instances of exploitation of pathogens for pest management and mass production techniques of pathogens (Safety and registration of microbial pesticides).

UNIT IV
Mammalian toxicity and safety of insect pathogens. Safe use of insect pathogens in integrated management of insect pests.

UNIT V
Biopesticides formulations, commercial production and economics.

Practicals
Laborator y practice, introduction to the microscope, ase ptic techniques, preparation of microbiological media, Culturing and isolation techniques of insect pathogens, smear preparation, Bacteria, gram’s staining, spore staining, Spore counting. Isolation of Bacteria from soil/diseased larvae, isolation of genomic DNA from Bt isolates, PCR analysis of Cry Genes of Bacillus thuringiensis isolates, resolving PCR product on agarose gel electrophoresis, enumeration of NPV using a standard haemocytometer, production of nuclear polyhedrosis, extraction of POBs of NPV, bioassay with NPV, bioassay with Bt, isolation of Fungus from diseased insect, preparation of media for mass culture of fungi.

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