DESCRIPTION OF COURSES

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ENT 610 INSECT GENETICS AND MOLECULAR BIOLOGY                                                                                 (2L+1P) II
(Inter disciplinary: Entomology with Genetics/molecular biology)

Objective
To impart knowledge to students on genetics, cytogenetics and molecular basis of insect behaviour.

Theory

UNIT I
Scope and importance of insect & molecular biology; Relevance to crop pest management.

UNIT II
Diversity of genetic systems in insects. Sex chromosome in Lepidoptera, Diptera, Coleoptera, Hemiptera and Hymenoptera. Genetic variations in populations; formation of biotype and sibling species; statistical techniques to study genetic variations in populations; parthenogenesis in insects, role of microorganisms such as Wolbachia.

UNIT III
Study of molecular techniques to address fundamental and applied entomological problems, including systematics. Marker-assisted selection of resistant genotypes of plants. Embryo rescue and related techniques in development of plant resistance to insects. Mechanisms of insecticide resistance – historical and contemporary.

UNIT IV

Insect transgenesis; risk assessment of genetically modified organisms, molecular biology of insect viruses (NPV, GV), genetic modifications in insect viruses for field efficacy; and advances in molecular biology of Bacillus thuringiensis.

Practicals
Study of insect polymorphism and other traits using facilities like PCR, Gel electrophorosis, genetic analysis related to insect resistance, development of insect population for genetic variability, bio- assays for event selection in insect population, study of chromosomes in insects and crossing methods and study of symbionts like Wolbachia in natural enemies.

Selected Readings