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GP 540 INTRODUCTION TO BIOINFORMATICS                                                                                 (3L+1P) I

Objective
To introduce basic concepts of bioinformatics

Theory

UNIT I
History and development of concept of bioinformatics; overview of protein and DNA sequences; sequences databases, retrieval and analysis; methods of sequence alignment - local, global pair wise and multiple alignment; Collection and storage of  sequences in the laboratory: DNA sequencing; genomic sequencing; cDNA libraries and sequencing cDNA; processing and submission of sequences; computer storage; sequence formats- Gen Bank, EMBL, NCBI, Stanford
University, etc.

UNIT II
Introduction to database management and DBMS. Introduction to Perl and Bioperl.

UNIT III
Phylogenetic prediction: Phylogeny and sequence variations; concept of evolutionary trees; methods in phylogeny-maximum parsimony, distance methods, maximum likelihood, reliability of prediction.

UNIT IV
Gene prediction: Gene structure and characteristics; ORF; methods for microbial and Eukaryotic gene predictions. Internet Resources.

UNIT V
Genome analysis: Genome structure and organization-Prokaryotes and Eukaryotes; sequence assembly and gene identification; methods - comparative genomics, proteomics; synteny, functional genomics.

Practicals
Sequence searching and alignment, writing programs in Perl for bioinformatics applications, Phylogenetic prediction, gene prediction

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