GP 600 DEVELOPMENT OF GENE CONCEPT                                                                                 (4L+0P) II

To give insight in to the dynamic concept of  gene, its structure, function and regulation of expression in prokaryotes and eukaryotes Pre-Mendelian concepts of gene: physical basis of heredity; Gene concept in classical genetics: Mendelian concepts of inheritance, gene interactions, recombination and linkage in lower and higher organisms, crisscross inheritance, gene mapping; Intra- and inter-genic complementation: complementation as test of allelism, complex loci, pseudoalleles.

Evolving concept of gene: Genetic control of metabolism, Garrod’s principle, Beadle and Tatums experiments; fine structure of gene- Beads-on-string, recombination within gene, between adjacent nucleotide, co-linearity principle: homoalleles and heteroalleles; genetic definition of  gene- complementation test as an operational definition, intragenic complementation, rII locus of bacteriophage T4, deletion mapping, gene-within-gene, overlapping gene, gene-protein relationship.

Chemical nature of gene: structure and properties of DNA and RNA; DNA, RNA and Prion as genetic material; Replication of  genetic material in prokaryotes and eukaryotes; Transfer of genetic information: Central dogma of  molecular biology,transcription and translation in prokaryotes and eukaryotes; reverse transcription,multiple factor hypothesis and quantitative traits, genetic code: deciphering and analysis of genetic code, nature and properties of genetic code, one code-two amino acids; Mutation: nature and molecular mechanism of mutation, intra- and inter-genic mutation suppression.

Regulation of gene expression in prokaryotes: inducible and repressible system, operon concept, attenuation, feedback inhibition; Regulation of gene expression in eukaryotes: level of gene regulation, gene activation through promoter-binding and enhancer-binding protein interaction, transcription regulation, post-transcriptional modifications including RNA processing, RNA editing, post-translational modifications; ncRNA,miRNA, siRNA, significance and ncRNA mediated regulation of gene expression, gene silencing, riboswitches.

Nuclear architecture and gene expression: nuclear compartments, chromatin domains, non-random nuclear architecture; Genetic basis of antibody diversity; alternate splicing; split gene: properties and evolution, Extra-chromosomal genes; Mobile genetic elements; Genetic regulation of virus, viroids, virusoids and prions; Genome complexity: C-value paradox, gene cluster, gene families.

Genes in population: Hardy- Weinberg law, molecular evolution, linkage disequilibrium.

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