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GP 604 INNOVATIVE APPROACHES IN PLANT BREEDING                                                                                 (2L+0P) III

Objective
To familiarize students with the latest in plant breeding approaches.

Theory

UNIT I
Introduction, Markers: morphological, isozymes, DNA markers (RFLP, RAPD, AFLP, SSR, SNP). Construction of linkage map; use of mapping populations (F2, RILs, NILs, back cross, doubled haploids)-applications, advantages, constraints. Applications of  molecular markers-finger printing, phylogenetic relationships. Tagging agronomically important traits. Assessing heterotic performance; Marker assisted selection (MAS) for oligogenic traits, MAS for QTLs. Gene pyramiding using molecular markers.

UNIT III
Transgenic plants-applications of transgenic technology, molecular farming, antisense RNA technology examples from published literature (crop quality, herbicide resistance, insect resistance, disease resistance, viral resistance)-organelle transformation, stability of transgenes, integration of transgenics in plant breeding.

UNIT IV
Biosafety issues of transgenics. Somatic hybridization applications and constraints. Somaclonal variation in crop improvement-overview and future prospects,

UNIT V
Plant genetic resources- characterization and utilization. Breeding for biotic stress resistance, abiotic stress tolerance and nutritional quality. Apomixis and its utilization.

UNIT VI
Functional markers, Reverse genetics approaches: Targeting Induced Local Lesions IN Genomes (TILLING), ECOTILLING and its application in crop breeding, Allele mining, Genome assisted breeding, Metabolomics assisted breeding, Overcoming domestication bottlenecks using molecular tools.

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