DESCRIPTION OF COURSES

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GP 620 APPLIED CYTOGENETICS                                                                                 (3L+1P) III

Objectives
This course aims to teach advanced techniques in manipulating cytogenetics for genome analysis in crop species.

Theory

UNIT I
Karyotyping – Chromosome banding and chromosome painting Tracking introgressions using FISH, GISH, loclalization and mapping of genes/genomic segments. – Distant hybridization - Role of polyploids in crop evolution and breeding - auto and allopolyploids.

UNIT II
Location and mapping of genes on chromosomes by various cytogenetical tools : Deficiency method, Interchanges, all arms marker method, linked marker method, Inversions, Telocentrics. Relative efficiency of different methods

UNIT III
Applications of  cytogenetical methods for crop improvement; balanced lethal systems, their maintenance and utility; multiple interchanges-use in producing inbreds, transfer of genes- linked marker methods;

UNIT IV
Duplication - production and use; inversions and location of  genes; B/A chromosome translocations and gene location Duplications- Production and use. Balanced tertiary trisomics- use in hybrid seed production. Polyploidy induction methods: Use of auto-polyploids. Haploidy- methods of production and use.

UNIT V
Trisomics- different types, production, breeding behaviour and location of genes using trisomics. Monosomics -methods of production, inter varietal substitutions, allelic and non-allelic interactions.

UNIT VI
Gene transfer by distant hybridization: scope and limitation, methods to overcome barriers- tissue culture. Allopolyploids-synthesis of  new crop species and varieties, Alien chromosome addition and substitutions. Chromosomal control of meiotic pairing and induced transfer of alien genetic variation.

Suggested Readings