DESCRIPTION OF COURSES

Close

MB 502 SOIL MICROBIOLOGY-I                                                                                 (3L+2P) I

Objective
To teach the basic concepts related to soil environment (microbial cycling of elements, diversity in microbial functions, microbial interactions, plant growth promoting microorganisms: conventional and molecular methods of microbial identification and enumeration-role of microorganisms in productive systems and ecological health)

Theory

UNIT I
Soil microorganisms: Major groups, their diversity, abundance, characteristics .

UNIT II
Direct and indirect methods of studying soil microorganisms and their activities: Dilution plate count method, Burried slide technique, G+C estimate, MPN method, Staining procedures and identification aided by microscopy, Molecular methods of  microbial diversity analysis : RISA,TGGE, DGGE, T-RFLP, BIOLOG, FAME analysis.

UNIT III
Carbon cycle, Decomposition of organic matter, Dynamics of micro-organisms during formation of soil organic matter, Humus and fulvic acid, Quantity and distribution of organic matter in soil.

UNIT IV
Biodegradation of starch, Cellulose, Hemicellulose, Pectin and lignin in soil , Nitrogen cycle: Ammonification, Nitrification, Denitrification, Non-symbiotic and symbiotic nitrogen fixation through bacteria and nitrogen assimilation, Soil health, Major microbial indicators and their significance.

Practicals
Quantification of total bacterial, fungal and actinomycetes counts from soils by pour & spread plate method under contrasting agronomic use, Assessment of soil microbial activity by soil respiration, dehydrogenase and FDA- hydrolysis analysis, Evaluation of cellulase activity of soil microflora in soils with and without amendment with crop residues, Evaluation of the amylolytic activity of soil microflora and its comparison with known (standard) microorganisms, Study of the decomposition rate of different agricultural residues in soil by carbon dioxide evolution method, Fractionation of soil organic matter content (Humus), Estimation of ammonia oxidizing bacteria and nitrite oxidizing bacteria from a soil sample using most probable number (MPN) technique,Isolation of free living nitrogen fixing bacteria from a soil sample.

Suggested Readings