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MB 508 SOIL MICROBIOLOGY-II                                                                                 (3L+1P) III

Objective
To teach the basic concepts of plant-microbe interactions.

Theory

UNIT I
Plant-microbe interactions, Endophytic and pathogenic interactions, Rhizosphere, Rhizoplane, Spermosphere and phyllosphere, Root exudates, Quorum-sensing in bacteria, Flow of signals in response to carbon substrates.

UNIT II
Legume –Rhizobium symbiosis, Frankia- Actinorhizal symbioses, Classification of nodulating bacteria, Formation of nodules in leguminous plants, Types of nodules, Genetics of nodulation and nitrogen fixation, sym genes, nod genes, nif genes and fix genes, NOD factors, Hydrogenases

UNIT III
Type three secretion systems, Plant growth promoting rhizobacteria (PGPR) and their direct and indirect mechanism of action, Biocontrol agents and their action mechanism.

UNIT IV
Mycorrhizae, Types of mycorrhizae and their interactions with plants, Biochemical/ molecular aspects.

Practicals
R:S ratio in soil & endorhizosphere, Isolation of rhizobia from root nodules, Collection and TLC analysis of root exudates for amino acids, Isolation and enumeration of phyllosphere microflora, Estimation of nitrogenase activity of rhizobia, Isolation of PGPR from soil ( N2 fixers, P-solubilizers, siderophore producers, HCN producers, antibiosis), MPN estimation of rhizobia from soil, % colonization of VAM in roots, Intrinsic antibiotic resistance pattern in rhizobia, Estimation of IAA production in microbes, PCR for microbial antibiotics

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