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MB 607 MICROBIAL GENETICS                                                                                 (3L+1P) II

Objective
To teach the concepts related to the genetics of micro-organisms.

Theory

UNIT I
Principles of microbial genetics, Basic procedures and terminology, Cis-trans complementation, Genome organization in bacteria and viruses, Gene transfer, Transformation, Conjugation, Transduction and methods of  gene mapping, Transposons and insertion sequences, Extra- chromosomal genetic elements, Plasmids and their inheritance, Genetic analysis of bacteriophages and cyanophages.

UNIT II
Gene, Genetic code, Operon concept and its regulation, Gene regulation and expression, Genetic engineering, Restriction enzymes, Vectors, Cloning genomic literary.

UNIT III
Recombinant DNA and mechanisms of recombination, Applications of rDNA, Safety issues and ethics in using rDNA, DNA replication, DNA Polemerases, Type of replication, Mutation and repair mechanisms, Protein synthesis and central dogma, Nucleic acid synthesis and sequencing.

Practicals
Isolation of  genomic DNA and plasmid DNA, Curing of  plasmid, Inducing mutation using chemical/UV rays (mutagenesis and isolation of mutants), Isolation of bacteriophage, One step growth experiment with λ phage, Preparation of  genomic library and competent cells, PCR amplification and cloning of the PCR product, Conjugation and transformation experiments.

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