DESCRIPTION OF COURSES
MB 607 MICROBIAL GENETICS (3L+1P) II
To teach the concepts related to the genetics of micro-organisms.
Principles of microbial genetics, Basic procedures and terminology, Cis-trans complementation, Genome organization in bacteria and viruses, Gene transfer, Transformation, Conjugation, Transduction and methods of gene mapping, Transposons and insertion sequences, Extra- chromosomal genetic elements, Plasmids and their inheritance, Genetic analysis of bacteriophages and cyanophages.
Gene, Genetic code, Operon concept and its regulation, Gene regulation and expression, Genetic engineering, Restriction enzymes, Vectors, Cloning genomic literary.
Recombinant DNA and mechanisms of recombination, Applications of rDNA, Safety issues and ethics in using rDNA, DNA replication, DNA Polemerases, Type of replication, Mutation and repair mechanisms, Protein synthesis and central dogma, Nucleic acid synthesis and sequencing.
Isolation of genomic DNA and plasmid DNA, Curing of plasmid, Inducing mutation using chemical/UV rays (mutagenesis and isolation of mutants), Isolation of bacteriophage, One step growth experiment with λ phage, Preparation of genomic library and competent cells, PCR amplification and cloning of the PCR product, Conjugation and transformation experiments.