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NEM 507 FUNDAMENTALS OF NEMATODE PHYSIOLOGY                                                                                 (2L+1P) I

Objective
  To understand the basic mechanism of host finding, metabolic pathways, growth and development in nematodes.

Theory

UNIT I
  Principles of physiology. History and importance of nematode physiology in management of plant parasitic nematodes.

UNIT II
  Cell structure, Cell organelles, structure of cell organelles, Physiological functions of cell organelles; Chemical composition of nematodes. Pseudocoelomic fluid and its function.

UNIT III
  Host finding mechanism, Feeding mechanisms and physiology, Hydrolytic enzymes of nematodes and their role in physiopathology of host.

UNIT IV
  Metabolism of carbohydrates, proteins and fatty acids; Physiology of digestion; Physiology of excretion, osmoregulation, permeation dynamics, steroid and hormonal regulation in nematodes

UNIT V
  Physiology of growth, development, reproduction, molting and hatching

Practicals
  Solutions, buffers, Isolation of nucleic acids and proteins from plants and nematode juveniles, quantification of nucleic acids and proteins from plants and nematode juveniles, molecular weight estimation of nucleic acids and proteins on agarose and polyacrylamide gels, RFLP of plant and nematode DNA, RAPD of nematode and plant DNA using PCR technique

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