DESCRIPTION OF COURSES

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PGR 503 PRINCIPLES AND METHODS OF GERMPLASM CONSERVATION                                                      (2L+1P) III

Objective
To impart knowledge on crop germplasm conservation with particular emphasis on seed genebanks.

Theory

UNIT I
In situ and ex situ conser vation: concept of  biosphere reserves, gene sanctuaries, on-farm conservation, field genebanks, botanical gardens, herbal gardens, in vitro repositories and cryo- banks.

UNIT II
Short-, medium- and long-term conservation, concept of base, active and working collections, seed structure and function, physiological and genetic changes during storage, theories of aging, viability equations, predicting storage life of seeds, dormancy and germination.

UNIT III
Genebank management: acquisition, accessioning and processing of germplasm samples for storage, genebank standards for various crops, ISTA, AOSA, IPGRI guidelines, monitoring and regeneration of plant germplasm.

UNIT IV
Design of storage facilities, maintenance and operation of storage modules.

UNIT IV
Information management in genebanks, strategies for revival and rescue of rare genetic material.

Practicals
Seed structure and morphology; seed germination and seedling evaluation; seed viability test, seed sampling and purity analysis, seed dormancy and dormancy breaking treatments, moisture testing methods, vigour testing methods and seed leachate analysis, accelerated aging of seeds and their assessment, seed processing and storage in Gene Bank.

Suggested Readings