DESCRIPTION OF COURSES
PGR 505 BIOTECHNOLOGY IN PLANT GENETIC RESOURCE MANAGEMENT (3L+2P) III
To provide information on use of biotechnology in germplasm conservation including molecular characterization and conservation technologies.
Tissue culture and PGR conservation, Tissue culture media, Sterilization techniques, In vitro clonal multiplication, Somatic embryogenesis, Meristem culture and virus elimination, In vitro conservation –Introduction and principle, Strategies for in vitro short- and medium-term conservation, In vitro collection, in vitro exchange of germplasm.
Plant Cryopreservation-Introduction, Principle of cryotolerance, Techniques of cryopreservation: slow cooling, desiccation, pregrowth, encapsulation-dehydration, vitrification, droplet freezing, Cryoprotectants, Cryopreservation of seeds and pollen, Cryopreservation of in vitro cultures, Application of in vitro cryopreservation techniques in monocots and dicots - case studies, Management of in vitro and cryobanks, Genetic stability of in vitro conserved and cryopreserved germplasm, Importance of database for in vitro and cryopreserved germplasm.
The need for plant germplasm characterization; introduction to different techniques for plant germplasm characterization; biochemical and molecular marker techniques for germplasm characterization and evaluation; recent advances in molecular genetic diversity analysis –use of SNPs and microarrays; data handling and statistical analysis; analysis of genetic diversity; marker assisted evaluation of core collections; conservation of genomic resources; molecular markers for gene bank management; detection of adventitious transgenics.
- Preparation of stock solutions; media preparation; preparation of explants and culture initiation in monocots and dicots; meristem isolation and culture establishment; subculture of shoots in monocots and dicots, hardening and field establishment of plantlets; preparation of cryoprotectant solutions and regrowth media; cryopreservation of in vitro cultures- isolation of explants and pretreatment.
- Encapsulation-dehydration technique; encapsulation-vitrificaion technique; assessing genetic stability of in vitro conserved and cryopreserved germplasm.
- Basic techniques in molecular biology; isolation of Genomic DNA from leaves; purification of DNA Quantification of DNA; PCR; RAPD, ISSR, demonstration of RFLP, AFLP, STMS; data Handling and Statistical Analysis.
- Ashmore, S.E. 1997. Status Report on the Development and Application of In Vitro Techniques for the Conservation and Use of Plant Genetic Resources. IPGRI, Rome.
- Benson, E.E. (ed.) 1999. Plant Conservation Biotechnology. Taylor & Fancis Ltd. London. Bhojwani, S.S. and Razdan, M.K. 1983. Plant Tissue Culture: Theory and Practice. Elsevier Science Publishing Co.Inc. New York.
- Dodds, J.H. (eds.) 1991. In Vitro Methods for Conservation of Plant Genetic Resources. Chapman and Hall, London.
- Dodds, J.H. and Robberts, L.W. 1985. Experiments in Plant Tissue Culture, 2nd Ed., Cambridge University Press, Cambridge, London.
- Engelmann, F. and Takagi, H. (eds) 2000. Cryopreservation of Tropical Plant Germplasm - Current Research Progress and Application, IPGRI, Rome/JIRCAS/Japan.
- Ford-Lloyd, B.V., Newburry, J.H. and Callow, J.A. (eds.) 1998. Biotechnology and Plant Genetic Resources: Conservation and Use. CABI, Wellingford.
- Griffin, H.G. and Griffin, A.M. 1994. PCR Technology: Current Innovations. CRC Press, London. Henry, R.J. (Editor) Plant Genotyping: The DNA Fingerprinting of Plants, Publisher: CABI Publishing. Karp, A., Isaac, P.G. and Ingram, D.S. 1998. Molecular Tools for Screening Biodiversity – Plants and Animals. Chapman and Hall, London.
- Kartha, K.K. (ed) 1995. Cryopreservation of Plant Cells and Organs. CRC Press, Boca Rataon, Florida. Sambrook, J., MacCallum, P., Russell, D.(eds.) 2001. Molecular Cloning: A Laboratory Manual (Third Edition), CSHL Press.