DESCRIPTION OF COURSES

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PGR 505 BIOTECHNOLOGY IN PLANT GENETIC RESOURCE MANAGEMENT                                    (3L+2P) III

Objective
To provide information on use of biotechnology in germplasm conservation including molecular characterization and conservation technologies.

Theory

UNIT I
Tissue culture and PGR conservation, Tissue culture media, Sterilization techniques, In vitro clonal multiplication, Somatic embryogenesis, Meristem culture and virus elimination, In vitro conservation –Introduction and principle, Strategies for in vitro short- and medium-term conservation, In vitro collection, in vitro exchange of germplasm.

UNIT II
Plant Cryopreservation-Introduction, Principle of cryotolerance, Techniques of cryopreservation: slow cooling, desiccation, pregrowth, encapsulation-dehydration, vitrification, droplet freezing, Cryoprotectants, Cryopreservation of seeds and pollen, Cryopreservation of in vitro cultures, Application of  in vitro cryopreservation techniques in monocots and dicots - case studies, Management of in vitro and cryobanks, Genetic stability of in vitro conserved and cryopreserved germplasm, Importance of database for in vitro and cryopreserved germplasm.

UNIT III
The need for plant germplasm characterization; introduction to different techniques for plant germplasm characterization; biochemical and molecular marker techniques for germplasm characterization and evaluation; recent advances in molecular genetic diversity analysis –use of SNPs and microarrays; data handling and statistical analysis; analysis of genetic diversity; marker assisted evaluation of core collections; conservation of genomic resources; molecular markers for gene bank management; detection of adventitious transgenics.

Practicals

Suggested readings