DESCRIPTION OF COURSES

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PGR 602 IN VITRO CONSERVATION AND CRYOPRESERVATION                                                                               (2L+2P) II

Objective
To equip students with skills in using tissue culture and cryopreservation techniques for conservation and management of PGR.

Theory

UNIT I
In vitro techniques in PGR management; in vitro clonal propagation for germplasm conservation; somaclonal variation and its implication in PGR conservation; in vitro collecting; meristem culture, virus indexing and elimination, in vitro exchange and its international guidelines.

UNIT II
Techniques of in vitro conservation of tropical and temperate crops under slow/normal growing conditions; tissue culture in circumventing crossability barriers embryo rescue technique, rescue and survival of rare and endangered species; management of large in vitro collections; concept of active and base in vitro genebanks.

UNIT III
History of plant cryopreservation; structural, physiological and biochemical basis of desiccation and freezing sensitivity of plant cells, tissues and organs; cryoprotectants and their mode of action, factors affecting cryoprotection.

UNIT IV
Methods of cryopreservation – conventional slow cooling and vitrification-based methods; factors influencing success in cryopreservation importance of pre-treatments, pre-conditioning of explant donor plants, regrowth media and culture conditions; advances in cryogenic protocols; status of application of cryopreservation in PGR conservation, application in pharmaceutical industry, forest breeding and production of trangenics.

UNIT V
Monitoring genetic stability of in vitro-conserved and cryopreserved germplasm.

Practicals

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