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PGR 605 PRINCIPLES AND METHODS IN ANALYSES OF MOLECULAR DIVERSITY                                                           (2L+2P) II

Objective
To provide insight into organization and structure of genetic variation in plant populations and practical skills in molecular diversity analysis.

Theory

UNIT I
Organization and structure of genetic variation in natural populations, organization and evolution of eukaryotic genome.

UNIT II
Molecular verses other conventional methods for assessing genetic variation; sampling for molecular analyses, functional polymorphism, uses and applications of molecular markers in PGR - analysis of genetic diversity, identification of gaps in collection, evaluation and characterization etc.

UNIT III
Biochemical and molecular markers, molecular cytology, principles of RFLPs, PCR based techniques, AFLPs, SNPs and DNA sequencing; statistical treatment of the molecular data and interpretation; recent advances: emerging techniques, their applicability and uses.

Practicals

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