DESCRIPTION OF COURSES

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PL PATH 502 PLANT VIROLOGY                                                                                 (2L+1P) I

Objective
To provide knowledge in history of plant viruses, their biological properties, diagnostics and management

Theory

UNIT I
History of plant viruses, composition and structure of viruses.

UNIT II
Symptomatology of important plant viral diseases, transmission, chemical and physical properties, host virus interaction, virus vector relationship.

UNIT III
Virus nomenclature and classification, Structure of plant virus, genome organization, replication and movement of viruses.

UNIT IV
Isolation and purification, estimation of virus titre and purity, electron microscopy, protein and nucleic acid based diagnostics.

UNIT V
Mycoviruses, phytoplasma arbo and baculoviruses, satellite viruses, satellite RNAs, phages, viroids, prions.

UNIT VI
Origin and evolution, mechanism of resistance, genetic engineering, ecology, and management of plant viruses.

Practicals
Study of symptoms caused by viruses, transmission, assay of viruses, physical properties, purification, method of raising antisera, serological tests, electron microscopy, spectrophotometry and ultratomy, PCR, preservation of virus specimens, preparation of herbrium

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