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PL PATH 503 PLANT BACTERIOLOGY                                                                                 (2L+1P) I

Objective
To provide basic knowledge on biology, classification and nomenclature, survival, preservation of phytopathogenic prokaryotic bacteria, phytoplasma, bdelbovibrios and bacteriophages.

Theory

UNIT I
History of bacteriology, nomenclature and classification of bacteria, bacteriophages-morphology, types and uses, mycoplasma and bdellovibrios

UNIT II
Bacterial cell-morphology, organelles and their functions, cell wall structure and chemistry, endospore and its formation, composition and function, flagellar structure, arrangements, movements

UNIT III
Growth, nutrition requirements, reproduction, preservation of bacterial cultures and variability among phytopathogenic procarya.

UNIT IV
Plasmids, bacterial conjugation, transduction and transformation

UNIT V
Important bacterial diseases: Bacterial leaf blight of rice, bacterial blight of pomegranate, cotton bacterial blight, bacterial wilt of solanaceous vegetables, soft rot of vegetables and black rot of crucifers.

UNIT VI
Management strategy for bacterial diseases, survival and dissemination of bacteria.

Practicals
Isolation, purification, identification and host inoculation of phytopathogenic bacteria, staining methods, biochemical and serological characterization, isolation of plasmid and use of antibacterial chemicals/antibiotics

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