DESCRIPTION OF COURSES

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PL PATH 505 DETECTION AND DIAGNOSIS OF PLANT DISEASES                                                 (1L+2P) I

Objective
To impart knowledge on various methods/techniques/instruments used in the study of plant diseases/pathogens.

Theory

UNIT I
Biological, serological and nucleo-based techniques for the detection of virus and virus like pathogens,

UNIT II
Fungal disease diagnosis-symptomatology, collection of samples and their preservation, Koch’s postulates, isolation techniques, purification and single spore isolation, inoculation technique and creation of artificial epiphytotics of fungal pathogens, long term storage and preservation of fungal cultures, molecular detection of fungal pathogens, data collection, compilation and scientific writing

UNIT III
Symptoms of bacterial diseases and characteristics of  phytopathogenic bacteria, isolation of phytopathogenic bacteria from rhizosphere and phyllospheres, bacterial stains and staining methods, techniques for purification and pathogenecity

Practicals
Methods to prove Koch’s postulates with biotroph and necrotroph pathogens, pure culture techniques, use of selective media to isolate pathogens, preservation of plant pathogens and disease specimens, use of haemocytometer, micrometer, centrifuge, pH meter, camera lucida. Microscopic techniques and staining methods, phase contrast system, chromatography, use of  electron microscope, spectrophotometer, ultracentrifuge and electrophoretic apparatus, disease diagnostics, serological and molecular techniques for detection of plant pathogens. Evaluation of fungicides, bactericides etc.; field experiments, data collection and preparation of references.

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