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PL PATH 602 ADVANCED VIROLOGY                                                                                 (2L+1P) II

Objective
To provide knowledge in recent advancement in study of plant viruses and their management

Theory

UNIT I
Introduction to Advanced Virology Mechanism of virus transmission by vectors, virus-vector relationship, bimodal transmission and taxonomy of vectors and viruses, vector specificity for classes of viruses, virus replication, assembly and architecture, ultrastructural changes due to virus infection, variation, mutation and virus strains.

UNIT II
Production PAb and hybridoma, nucleo-based diagnostic technique, Immunoglobulin structure and functions of various domains, methods of immunodiagnosis, hybridoma technology and use of monoclonal antibodies in identification of viruses and their strains, Polymerase Chain Reaction, Rolling Circle replication.

UNIT III
Genome organization, gene expression in Gemini viruses, mechanism of replication, transcription and translational strategies of pararetroviruses and gemini viruses, satellite viruses and satellite RNA genome organization in tobamo-, poty-, bromo, cucummo, ilar and tospoviruses.

UNIT IV
Gene expression and regulation, viral promoters, molecular mechanism of host virus interactions, virus induced gene, molecular mechanism of vector transmission, symptom expression, viroids and prions.

UNIT V
Genetic engineering with plant viruses, viral suppressors, a RNAi dynamics, resistant genes. Viruses potential as vectors, genetically engineered resistance, transgenic plants.

UNIT VI
Techniques and application of tissue culture. Origin, evolution and interrelationship with animal viruses.

Practicals
Purification of virus(es), SDS-PAGE for molecular weight determination, production of polyclonal antiserum, purification of IgG and conjugate preparation, serological techniques (i) DAC-ELISA (ii) DAS -ELISA (iii) DIBA (iv) Western blots (v) (ab) 2-ELISA, vector transmission (one each with aphid, leaf hopper and whitefly), methods for collecting vectors and their maintenance, nucleic acid isolation, DOT-blot, southern hybridization, probe preparation and autoradiography, PCR application and viral genome cloning, sequencing annotation of genes.

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