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PL PATH 603 ADVANCED BACTERIOLOGY                                                                                 (2L+1P) II

Objective
To provide advanced knowledge on characterization, identification of bacteria, pathogenesis, mechanism of disease development and recent approach in bacterial disease management.

Theory

UNIT I
Nomenclature, characteristics and classification of bacteria, Ultrastructures and biology of bacteria.

UNIT II
Fastidious bacteria, mechanism of soft rot (Erwinia spp.) development, mechanism of crown gall formation (Agrobacterium tumefaciens), Mechanism of wilt (Ralstonia solanacearum) development.

UNIT III
Role of enzyme, toxin, expolysaccharide, polypeptide signals in disease development, epidemiology in relation to bacterial plant pathogens

UNIT IV
Host-bacterial pathogen interaction, quorum-sensing phenomenon, Type III secretion system, HR/ SR reactions, R-genes, Avr-genes, hrp genes, effector protein, survival, colonization ability of bacteria, bacterial EPS and their role as disease determinant

UNIT V
Plasmid biology, molecular variability among phytopathogenic procarya and possible host defense mechanism(s), genetic engineering for management of bacterial plant pathogens-gene silencing, RNAi technology.

UNIT VI
Polyclonal and monoclonal antibodies against phytopathogenic bacteria, PCR based detection of plant pathogenic bacteria, use of  advanced techniques in quarantine, PRA, development of diagnostic kit.

UNIT VII
Beneficial prokaryotes- Endophytes, PGPR, phylloplane bacteria and their role in disease management, endosymbionts for host defence.

Practicals
Pathogenic studies and race identification; plasmid profiling of bacteria; fatty acid profiling of bacteria; RAPD prolfiling of bacteria and variability status; Endospore, Flagiler staining; test for secondary metabolite production, cyanides, Extracellular Polymeric Substance, siderophore; specific detection of phytopathogenic bacteria using species/pathovar specific primers, basic techniques in diagnostic kit development, molecular tools to identify phytoendosymbionts

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