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PL PATH 604 MOLECULAR BASIS OF HOST PATHOGEN INTERACTION                                                                                 (2L+1P) III

Objective
To provide knowledge on host pathogen interaction and its application at molecular level.

Theory

UNIT I
Importance and role of biotechnological tools in Plant Pathology- Basic concepts and principles to study host pathogen relationship.

UNIT II
Molecular basis of host-pathogen interaction- fungi, bacteria and viruses; recognition system, signal transduction.

UNIT III
Induction of defense responses- pathogenesis related proteins, HR, reactive oxygen species, phytoalexins and systemic acquired resistance, Programmed Cell Death, Viral induced gene silencing.

UNIT IV
Molecular basis of gene-for-gene hypothesis; R-gene expression and transcription profiling, mapping and cloning of resistance genes and marker-aided selection, pyramiding of R genes.

UNIT V
Biotechnology and disease management; development of disease resistance plants using genetic engineering approaches, different methods of gene transfer, biosafety issues related to GM crops.

Practical
Protein, DNA and RNA isolation, Plasmids extraction, PCR analysis, DNA and Protein electrophoresis, bacterial transformation.

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