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PP 504 HORMONAL REGULATION OF PLANT GROWTH AND DEVELOPMENT                                                                                 (3L+1P) II

Objective
  To apprise the students about structure and function of plant growth regulators.

Theory

UNIT I
  Definition and classification of plant growth regulators- Hormones, endogenous growth substances and synthetic chemicals, Endogenous growth regulating substances other than hormones. Tricontanol, phenols – polyamines, jasmonates, concept of death hormone.

UNIT II
  Site of  synthesis, biosynthetic pathways and metabolism and the influence on plant growth development of individual group of hormones- Auxins, Gibberellins, Cytokinins, Abscisic acid, Ethylene and Brassinosteroids. Hormone mutants and transgenic plants in understanding role of hormones.

UNIT III
  Signal perception. Transduction, and effect at functional gene level of different hormones- Auxins- cell elongation, Gibberellins - germination of dormant seeds, Cytokinins- cell division. Retardation of senescence of plant parts, Abscisic acid-stomatal closure and induction of drought resistance, Ethylene- fruit ripening. Interaction of hormones in regulation of plant growth and development processes.

UNIT IV
  Rooting of cuttings-Flowering. Apical dominance, molecular aspects of control of reproductive growth and development. Synthetic growth regulators- Classification, their effect on plant growth and development. Practical utility in agriculture and horticulture.

Practicals
  Quantification of  hor mones- Principles of  bioassays, physico chemical techniques and immunoassay, Extraction of hormones from plant tissue.
   Auxins- bioassays- auxins effect on rooting of cuttings, abscission, apical dominance, Gibberellins- bioassays-GA effect on germination of dormant seeds, Cytokinin- bioassays- estimation using immunoassay technique, cytokinin effect on apical dominance and senescence, ABA bioassays estimation using immunoassay technique. ABA effect on stomatal movement, Ethylene bioassays, estimation using physico- chemical techniques- effect on breaking dormancy in sunflower and groundnut.

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