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SSAC 509 RADIO TRACER TECHNIQUES IN SOIL AND PLANT STUDIES                                                         (3L+1P) I

Objective
To train the students in the use of radiotracer techniques in soil-plant studies including assessment of soil fertility, nutrient movement in soil and their absorption by plants

Theory

UNIT I
Radioactivity - discovery, nature and properties of radiations; atomic structure and units of radioactivity; radioisotopes - properties and decay principles; artificial radioactivity, nature and properties of nuclear reactions.

UNIT II
Interaction of radiations with matter; radiation hazards, dosimetry, safety procedures and waste disposal; statistics of counting.

UNIT III
Principles of tracer methodology; principles and use of  radiation monitoring instruments - proportional, Geiger Muller counter, solid and liquid scintillation counters, neutron moisture meter, mass spectrometry and radioassay.

UNIT IV
Use of stable isotopes; application of isotopes in ion transport, nutrient transformations; labeling, synthesis of labeled compounds; radio-activation and radiometric analysis.

Practicals

Storage and handling of radioactive materials; familiarization with counting equipments; determination of instrument efficiency of GM counter; determination of half life and decay constant, backscattering, self-absorption and E-max of beta radiations; preparation of soil and plant samples for radioactive measurements, and radioactively-labeled fertilizers; setting up of experiments on fertilizer use efficiency using radioisotopes; determination of A, E and L values of soil using 32P/ 65Zn; use of neutron probe for moisture estimation; measurement of 14C and 3H by liquid scintillation counting; single channel analyzer for determination of 65Zn; autoradiographic techniques; identification of radionuclides; statistics of radioactive measurements; de-contamination of surfaces.

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