DESCRIPTION OF COURSES

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SST 603 TESTING FOR GENUINENESS AND PURITY OF CULTIVERS                                                                               (2L +2P) II

Objective
To provide hands-on training on various field and laboratory methods of testing cultivar purity.

Theory

UNIT I
Objective of cultivar purity test, general principles and methods involved. Use and limitations of laboratory, green house and field plot methods in determination of genuineness of cultivars; a case study in hybrid cotton, reporting of results and inference.

UNIT II
Chemical-biochemical tests for species and cultivar purity: phenol test, seed and seedling tests, electrophoretic analysis of seed protein, isozymes etc, use of chromatography for analysis of secondary compounds etc.

UNIT III
DNA finger printing (RAPD, SSR, AFLP etc) and their use in varietal purity testing and registration of new varieties.

UNIT IV
Use of computer-based machine vision (MVT) for varietal identification and purity testing

Practicals
Chemical and biochemical tests for species and cultivar purity: phenol test, seed and seedling tests, electrophoretic analysis of seed protein and isozymes, DNA fingerprinting using PCR techniques, use of chromatography for analysis of secondary compounds.

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